09/09/2020: The Kelch lab, in collaboration with the
Sep 14, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA).
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Structure of the human clamp loader bound to the …
Structure of the human clamp loader reveals an
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How a DNA Polymerase Clamp Loader Opens a Sliding Clamp
Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of adenosine triphosphatases (ATPases). We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA.
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Structural analysis of a eukaryotic sliding DNA clamp
Jun 17, 2004 · Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell
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Structure of the human clamp loader reveals an
Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a …
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Structure of the human clamp loader reveals an
Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch
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6vvo.1 | SWISS-MODEL Template Library
Structure of the human clamp loader (Replication Factor C, RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen, PCNA) Coordinates PDB Format Method ELECTRON MICROSCOPY Oligo State heteroKefidKefidKefid-mer Ligands 4 …
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Structure of the human clamp loader bound to the sliding
Structure of the human clamp loader reveals an
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How a DNA polymerase clamp loader opens a sliding clamp.
Figure 2. Architecture of the T4 clamp loader/clamp/DNA complex A) Structure of the T4 clamp loader bound to an open clamp. Ribbon and schematic diagrams of the complex between the T4 clamp loader (multi-colored), the open T4 clamp (gray), which is broken between subunits I and III, and primer-template DNA.
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Structure of the human clamp loader bound to the …
Feb 18, 2020 · In order to visualize how the clamp loader interacts with the sliding clamp, we formed a complex of hRFC with PCNA and the Figure 1. Human clamp loader (hRFC) composition and function. (A) hRFC consists of five different AAA+ ATPase subunits, named A to E. Each subunit consists of an ATPase module and a collar domain.
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Jacob LANDECK | Graduate Student | University of
Structure of the human clamp loader bound to the sliding clamp: a further twist on AAA+ mechanism Structure of the human clamp loader bound …
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6vvo.1 | SWISS-MODEL Template Library
Structure of the human clamp loader (Replication Factor C, RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen, PCNA) Coordinates PDB Format Method ELECTRON MICROSCOPY Oligo State heteroKefidKefidKefid-mer Ligands 4 …
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Kelch Lab Publications Biochemistry & Molecular Pharmacology
May 14, 2021 · Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch. Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA.Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. Epub 2020 Sep 9. A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry.
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Clamp loader ATPases and the evolution of DNA replication
Apr 20, 2012 · A recently determined structure of the T4 bacteriophage clamp loader bound to an ATP analog and in complex with the sliding clamp and primer template DNA revealed a state of the system that we have long sought to visualize: an open clamp encircling DNA while in complex with an ATP-bound clamp loader. Another structure shows what happens when
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Structure of the human clamp loader reveals an
Structure of the human clamp loader reveals an
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DNA clamp - Wikipedia
A DNA clamp, also known as a sliding clamp or β-clamp, is a protein complex that serves as a processivity-promoting factor in DNA replication.As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand. The clamp-polymerase protein–protein …
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Brian A Kelch's lab
The human clamp loader Replication Factor C (RFC) and sliding clamp PCNA are both essential and play critical roles in several diseases. Despite decades of …
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RCSB PDB - 6VVO: Structure of the human clamp loader
we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a reaction intermediate prior to clamp opening and propose a confor-mational change necessary for activation, leading to a unique paradigm for the AAA+ ATPase mechanism. We examined
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Figure 14.1 from The RFC clamp loader: structure and
Fig. 14.1 Scheme of clamp loader function. ( a ) The structures of the E. coli β and human PCNA sliding clamps (PDB: 2POL and PDB: AXC, respectively). ( b ) Schematic of clamp loader function using eukaryotic RFC and PCNA as the example. ATP binding to RFC enables RFC to bind and open PCNA. In the presence of a primed template, RFC places PCNA onto DNA and then …
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"Structure of the human clamp loader bound to the sliding
The human clamp loader Replication Factor C (RFC) and sliding clamp PCNA are both essential and play critical roles in several diseases. Despite decades of study, no structure of human RFC has been resolved. Here, we report the structure of human RFC bound to PCNA by cryo-EM to an overall resolution of ~3.4 Å.
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